ProLine is a Biological Indicator system designed for use in the validation or
monitoring of sterilization cycles of tubing ranging from 1/8" to 5/8" tubing ID.
The ProLine PCD (Process Challenge Device) contains a filter paper disc
inoculated with bacterial spores of Geobacillus stearothermophilus (for Steam)
or Bacillus atrophaeus (for EO). The spore disc is packaged in a glassine
envelope and is located in the center of the ProLine housing. If adequate
sterilant penetration occurs, the spores on the disc will be deactivated and
verify an adequate sterilization cycle.
For a given length of tubing, the most difficult area to be sterilized
is the mid-point of the tubing length. This is where the ProLine device must be
placed. To do this, a representative piece of tubing should be cut at its
mid-point. The two half lengths should now be attached to the ProLine device by
slipping one end of each cut length of tubing over the tapered ends of the
ProLine device. The ProLine device is now located in the most difficult area of
the tubing to sterilize.
The ProLine system with its two attached lengths of tubing
should now be placed in the sterilizer along with a normal load being processed
where tubing would normally be included.
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Post Sterilization Testing
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Once the sterilization cycle is completed, the
ProLine and attached lengths of tubing are removed from the sterilizer chamber
and transferred to an area where an aseptic transfer of the spore disc to growth
media can be performed. To do this, grasp both sides of the ProLine device and
break the chamber open exposing the glassine packaged spore disc. The spore disc
must now be transferred aseptically to a tube of sterile TSB (Tryptic Soy Broth)
and incubated for Growth/No Growth testing. The opened ProLine device halves can
now be discarded as the device is a “single use only” product.
| Catalog # | Description | Units per box |
| PL-3-6-15 | Steam (log 6) | 15 |
| PL-1-6-15 | EO (log 6) | 15 |
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